9S00

X-ray structure of the alpha-L-fucosidase MfAlfDGH29 from Mariniflexile fucanivorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 61 2 2
Unit cell lengths	130.755, 130.755, 430.915
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.640 - 2.670
R-factor	0.1911
Rwork	0.189
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.20rc3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.640	2.810
High resolution limit [Å]	2.670	2.670
Number of reflections	63058	8988
<I/σ(I)>	17.3
Completeness [%]	100.0
Redundancy	40.2
CC(1/2)	0.999	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% (w/v) PEG 3,000 100 mM HEPES pH 7.5 for