9RYF
Heterodimeric ABC exporter TmrAB (wild type) in ATP-bound outward-facing occluded conformation in the absence of Mg2+
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.02 |
| Software | Topaz,cryoSPARC,PHENIX,EPU,Coot,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
