9RXY

Drt2, an Endogenised Ty1 Gag CA-CTD Restriction Factor, F323S Y329S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97948
Spacegroup name	C 1 2 1
Unit cell lengths	91.760, 38.833, 111.209
Unit cell angles	90.00, 96.65, 90.00

Refinement procedure

Resolution	45.570 - 3.184
R-factor	0.3017
Rwork	0.300
R-free	0.33910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.446
Data reduction software	XDS (Jan 10, 2022 (BUILT 20220110))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.461	3.535
High resolution limit [Å]	3.184	3.184
Rmeas	0.176	1.490
Rpim	0.069	0.576
Number of reflections	4022	202
<I/σ(I)>	7.197	1.203
Completeness [%]	59.2	11.05
Redundancy	6.4	6.63
CC(1/2)	0.997	0.534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	150 nL protein (45 mg/ml (3.88mM) in 20 mM Tris pH 8.5, 150mM NaCl, 1mM TCEP) mixed with 50 nL well solution (20%PEG3350, 0.1M BisTris Propane, pH7.5, 0.2M NaF)