9RXT
Structure of the PDZ1 domain from human NHERF1 with the C-terminal residues (NATRL) of the human sodium-dependent phosphate transporter 2A (NaPi-IIa, SLC34A1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-27 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967697 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 64.187, 64.187, 64.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.060 - 1.360 |
| R-factor | 0.1572 |
| Rwork | 0.155 |
| R-free | 0.19750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.943 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.383 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.125 | 3.780 |
| Rpim | 0.110 | 0.417 |
| Number of reflections | 19221 | 904 |
| <I/σ(I)> | 30.3 | 1.5 |
| Completeness [%] | 99.8 | 97.1 |
| Redundancy | 120 | 75.7 |
| CC(1/2) | 1.000 | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 100mM Bicine pH 9, 100mM NaCl, 30% PEG 550 MME |
