9RXR
Structure of the PDZ1 domain from human PDZK1 (NHERF3) with the C-terminal residues (VLKSTQF) of human URAT1 transporter (SLC22A12)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-11-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 35.887, 35.887, 72.069 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.080 - 1.690 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.466 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.100 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.156 | 1.958 |
| Rpim | 0.024 | 0.325 |
| Number of reflections | 9092 | 455 |
| <I/σ(I)> | 16.7 | 2.1 |
| Completeness [%] | 93.0 | 86.8 |
| Redundancy | 42.2 | 37 |
| CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES, pH 6.5, 700 mM NaCl, 18% PEG 4000 |
