9RX2
Crystal Structure of the Novel Fucanase MfFcnCGHnc from *Mariniflexile fucanivorans
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.110, 121.238, 148.244 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 49.530 - 2.920 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.645 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20rc3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.530 | 3.080 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 66133 | 9606 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.989 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% (w/v) PEG 4,000 100 mM Tris-HCl pH 8.5 |
