9RVI

10 micrometer HEWL crystals solved at room-temperature using fixed-target serial crystallography.

Replaces:  8PYQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SwissFEL ARAMIS BEAMLINE ESC
Synchrotron site	SwissFEL ARAMIS
Beamline	ESC
Temperature [K]	293
Detector technology	PIXEL
Collection date	2022-08-20
Detector	PSI JUNGFRAU 8M
Wavelength(s)	1.09
Spacegroup name	P 43 21 2
Unit cell lengths	78.700, 78.700, 38.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.820 - 1.470
R-factor	0.1492
Rwork	0.147
R-free	0.17240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.863
Data reduction software	CrystFEL (0.10.2)
Data scaling software	CrystFEL (0.10.2)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.350	1.500
High resolution limit [Å]	1.470	1.470
Number of reflections	21009	1018
<I/σ(I)>	10.95	2.91
Completeness [%]	100.0	100
Redundancy	530	363
CC(1/2)	0.990	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3	293	19.6% (w/v) NaCl, 5.6% (w/v) PEG 6000, 0.1 M NaAc pH 3.0