9RUE
Crystal Structure of the VS1-bound StARD3 Lipid-Binding Domain
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.873, 83.873, 82.309 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.460 - 2.088 |
| R-factor | 0.2074 |
| Rwork | 0.205 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | autoPROC (1.0.5) |
| Phasing software | BALBES (1.0.0) |
| Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.893 | 1.830 |
| High resolution limit [Å] | 1.753 | 1.753 |
| Rmerge | 0.113 | 0.722 |
| Rmeas | 0.117 | 0.746 |
| Rpim | 0.032 | 0.187 |
| Total number of observations | 399985 | |
| Number of reflections | 353233 | 1378 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 80.1 | |
| Redundancy | 14.5 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 0.1 M CHES pH 9.5, 0.2 M lithium sulfate, 0.3 M sodium potassium tartrate, 0.01 M DTT |
