9RSE
Human CD1d with lipid antigen bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9253 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.622, 156.373, 51.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.266 - 1.760 |
| Rwork | 0.213 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DIALS (3.21) |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | Servalcat (0.4.113) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.270 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmeas | 0.094 | 0.937 |
| Rpim | 0.049 | 0.487 |
| Number of reflections | 92489 | 3363 |
| <I/σ(I)> | 14.8 | 1.1 |
| Completeness [%] | 96.6 | 71.07 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.968 | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293.15 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium l-tartrate, 0.02 M sodium oxamate, 0.1 M bicine/Trizma base pH 8.5 |
