9RPL

Lactobacillus acidophilus SlpA self assembly domains I-II (aa 49-308)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-28
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	C 1 2 1
Unit cell lengths	149.069, 50.559, 118.638
Unit cell angles	90.00, 113.94, 90.00

Refinement procedure

Resolution	54.220 - 1.930
R-factor	0.2061
Rwork	0.204
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.628
Data reduction software	autoPROC (1.1.7)
Data scaling software	STARANISO (2.4.9)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.125	2.164
High resolution limit [Å]	1.929	1.929
Number of reflections	37108	1855
<I/σ(I)>	8.3
Completeness [%]	92.1
Redundancy	3.6
CC(1/2)	0.987	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 % PEG 3350, 50 mM sodium formate, 150 mM trisodiumcitrate; 4 g/l protein solution in 25 mM HEPES pH 7.5, 150 mM NaCl; 0.3 ul condition + 0.3 ul protein solution