9RNT

RIBONUCLEASE T1 WITH FREE RECOGNITION AND CATALYTIC SITE: CRYSTAL STRUCTURE ANALYSIS AT 1.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	48.730, 46.390, 41.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.500
R-factor	0.143
RMSD bond length	0.025
RMSD bond angle	0.052
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.500 *
Total number of observations	17624 *
Number of reflections	13115 *
Completeness [%]	84.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (%(w/v))
2	1	reservoir	PEG4000	35 (%(w/v))
3	1	reservoir	sodium acetate	5 (mM)
4	1	reservoir	calcium acetate	2 (mM)
5	1	reservoir	Tris acetate	20 (mM)