Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.055, 326.789, 88.101 |
| Unit cell angles | 90.00, 120.27, 90.00 |
Refinement procedure
| Resolution | 44.290 - 3.200 |
| R-factor | 0.2391 |
| Rwork | 0.237 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.184 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.290 | 3.317 |
| High resolution limit [Å] | 3.200 | 3.203 |
| Number of reflections | 70140 | 4668 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.940 | 0.418 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M Sodium Citrate pH 5 0.1M Magnesium Chloride hexahydrate 11% PEG 4000 20% Glycerol (Cryo) |
