9RKN
Crystal Structure of ACBI4-mediated ternary complex of KRAS G12R GCP with pVHL:ElonginC:ElonginB
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-24 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.303, 122.138, 80.328 |
| Unit cell angles | 90.00, 114.13, 90.00 |
Refinement procedure
| Resolution | 58.050 - 2.850 |
| R-factor | 0.2285 |
| Rwork | 0.226 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.628 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.050 | 2.952 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 29820 | 2920 |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.981 | 0.302 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.14 M Ammonium sulfate, 25% w/v Polyethylene glycol 3,350, 0.1M BIS-TRIS pH 5.8 |
