9RKJ

Crystal Structure of compound 3-mediated ternary complex of KRAS G12R GCP with pVHL:ElonginC:ElonginB

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00001
Spacegroup name	P 21 21 21
Unit cell lengths	101.602, 110.612, 114.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.500 - 2.890
R-factor	0.2399
Rwork	0.237
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.511
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.500	2.994
High resolution limit [Å]	2.890	2.890
Number of reflections	19250	259
<I/σ(I)>	7.3
Completeness [%]	65.1
Redundancy	2
CC(1/2)	0.977	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.1 M Bis Tris pH 7.3, 0.167 M trisodium citrate, 17%(w/v) PEG 3350