9RKC
Crystal Structure of ACBI4-mediated ternary complex of KRAS G12D C118S GDP with pVHL:ElonginC:ElonginB
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-24 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.915, 106.467, 82.742 |
| Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
| Resolution | 44.810 - 2.190 |
| R-factor | 0.2336 |
| Rwork | 0.232 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.775 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 2.273 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 59951 | 4959 |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 97.6 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.989 | 0.318 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Na2SO4, 15% w/v PEG 3,350, 0.1 M BIS-TRIS propane pH 7.79 |
