9RK8

Crystal Structure of compound 3-mediated ternary complex of KRAS G12V C118S GDP with pVHL:ElonginC:ElonginB

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00004 A
Spacegroup name	C 2 2 21
Unit cell lengths	96.189, 109.426, 251.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.180 - 2.630
R-factor	0.2331
Rwork	0.232
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.788
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.710	2.724
High resolution limit [Å]	2.630	2.630
Number of reflections	39396	190
<I/σ(I)>	9.12
Completeness [%]	72.8
Redundancy	10.2
CC(1/2)	0.998	0.217

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.1 M Tris pH 7.0, 0.2 M MgCl2, 10%w/v PEG 8000