9RK6

Paraplegin N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979515
Spacegroup name	C 2 2 21
Unit cell lengths	56.393, 63.844, 99.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.956 - 1.990
Rwork	0.239
R-free	0.29610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.455
Data reduction software	xia2 (0.5.898-g5f4e2fb3-dials-1.14)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.960	2.010
High resolution limit [Å]	1.990	1.990
Number of reflections	12698	474
<I/σ(I)>	11.7
Completeness [%]	99.9	62.9
Redundancy	7.2	8.3
CC(1/2)	0.999	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10mM NaCitrate, 15% Peg8k with seeds from 0.17M Ammonium sulfate, 25.5% PEG 4K, 15% glycerol