Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.800, 81.760, 51.720 |
| Unit cell angles | 90.00, 114.67, 90.00 |
Refinement procedure
| Resolution | 47.004 - 1.250 |
| Rwork | 0.151 |
| R-free | 0.17640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.815 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.004 | 1.304 |
| High resolution limit [Å] | 1.244 | 1.244 |
| Rmerge | 0.043 | 0.379 |
| Rpim | 0.027 | 0.258 |
| Number of reflections | 85942 | 4297 |
| <I/σ(I)> | 12.4 | 2.2 |
| Completeness [%] | 95.4 | |
| Redundancy | 3.4 | |
| CC(1/2) | 1.000 | 0.858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30mM sodium nitrate, 30mM disodium hydrogen phosphate, 30mM ammonium sulfate, 100mM MES-imidazole pH 6.5, 20%(w/v) PEG 550 MME, 10%(w/v) PEG 20K (Morpheus condition C1) |
