9RIC
Zn finger-finger DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-06 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1.3405 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.556, 56.054, 130.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.230 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.877 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.230 | 2.090 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.207 |
| Rmeas | 0.057 | 0.225 |
| Rpim | 0.021 | 0.085 |
| Number of reflections | 13221 | 3260 |
| <I/σ(I)> | 23.25 | 7.51 |
| Completeness [%] | 99.9 | 99.85 |
| Redundancy | 7.4 | 7 |
| CC(1/2) | 0.999 | 0.977 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1M NaCl 0.05M MES pH=6 10% PEG 2000 MME |
