9RHZ
Structure of a complex of biosynthetic proteins bE5 and CheytinYY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.880, 107.770, 58.890 |
| Unit cell angles | 90.00, 104.41, 90.00 |
Refinement procedure
| Resolution | 39.170 - 2.097 |
| R-factor | 0.2114 |
| Rwork | 0.210 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4 (10-JUL-2024)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmeas | 0.110 | 1.104 |
| Number of reflections | 36699 | 5860 |
| <I/σ(I)> | 10.15 | 1.74 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.68 | |
| CC(1/2) | 0.997 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M lithium acetate and 20%(w/v) PEG3350 |
