9RFW
Manikomycin bound to the Escherichia coli 50S ribosomal subunit
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOCONTINUUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.4 |
| Number of Particle | 483608 |
| Software | RELION,REFMAC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
