9RFB

Crystal Structure of Human Rac1 in Complex with the Scaffold Protein POSH (residues 321-348)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9655
Spacegroup name	P 43 21 2
Unit cell lengths	54.793, 54.793, 329.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.670 - 1.854
Rwork	0.195
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.412
Data reduction software	autoPROC (1.0.5 (20230222))
Data scaling software	autoPROC (1.0.5 (20230222))
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.670	2.027
High resolution limit [Å]	1.854	1.854
Rmerge	0.185	1.100
Rmeas	0.193	1.200
Rpim	0.052	0.389
Number of reflections	34144	1707
<I/σ(I)>	8	1.7
Completeness [%]	94.2	67
Redundancy	13.4
CC(1/2)	0.997	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.05 M phosphate, 20% (w/v) PEG 8000. RAC1 6 mg/mL , GMPPNP 1mM, POSH 3 mM