9RE8
Crystal Structure of the Protein-Kinase A catalytic subunit from Cricetulus griseus in complex with F138
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.913, 71.200, 97.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.570 - 1.500 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.20360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.570 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.073 | 1.051 |
| Number of reflections | 58411 | 2852 |
| <I/σ(I)> | 16.4 | 3.7 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.8 | 5.3 |
| CC(1/2) | 0.998 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM Mes/Bis-Tris, 75 mM lithium chloride, 1 mM DTT, 0.1 mM sodium EDTA, 0.25 mM Mega 8, 23% v/v methanol/water in reservoir |
