9RDC

Crystal structure of Phytophthora infestans effector AVRcap1b in complex with the ENTH domain of Nicotiana benthamiana NbTOL9a protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	85.874, 136.912, 195.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.570 - 4.100
R-factor	0.25005
Rwork	0.248
R-free	0.28596
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.529
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.570	4.490
High resolution limit [Å]	4.100	4.100
Rmerge	0.106	2.126
Rmeas	0.110	2.208
Rpim	0.031	0.595
Total number of observations	242580	59716
Number of reflections	18772	4401
<I/σ(I)>	9.6	1
Completeness [%]	100.0
Redundancy	12.9	13.6
CC(1/2)	1.000	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	NULL