9RCZ

Structure of the N-terminal domain of non-structural protein 1 (Nsp1) from SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	36.750, 36.750, 142.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.060 - 1.450
R-factor	0.1842
Rwork	0.183
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	1.540
High resolution limit [Å]	1.450	1.450
Number of reflections	33017	5385
<I/σ(I)>	20.3	1.6
Completeness [%]	99.8
Redundancy	6.78
CC(1/2)	1.000	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5 and 25% (w/v) PEG 3350. Reproducibility was improved by seeding.