9RCL
Laccase (multicopper oxidase) from Pediococcus pentosaceus 4618 mutant M455A-M456A soaked with Copper 10 minutes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 180 |
| Detector technology | PIXEL |
| Collection date | 2024-10-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.37786 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 123.590, 123.590, 179.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.510 - 2.200 |
| R-factor | 0.18613 |
| Rwork | 0.184 |
| R-free | 0.21705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.510 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.111 | 1.504 |
| Rmeas | 0.114 | 1.544 |
| Rpim | 0.026 | 0.346 |
| Total number of observations | 85350 | |
| Number of reflections | 81174 | 4403 |
| <I/σ(I)> | 18.7 | 2.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 19.5 | 19.4 |
| CC(1/2) | 0.999 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 85% 1 M Ammonium Sulfate, 0.1 M Sodium Acetate pH 4.6 and 15% of another condition with 0.2 Sodium Chloride, 0.1 M Phosphate-citrate pH 4.2 and 20% PEG 8000 |
