9RC8

Laccase (multicopper oxidase) from Pediococcus pentosaceus 4618 deletion 482-509 co-crystallized with Copper Chloride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	180
Detector technology	PIXEL
Collection date	2021-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97895
Spacegroup name	P 32 2 1
Unit cell lengths	124.363, 124.363, 182.238
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	107.700 - 3.200
R-factor	0.1619
Rwork	0.159
R-free	0.20979
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.700	3.390
High resolution limit [Å]	3.200	3.200
Rmerge	0.299	1.137
Rmeas	0.316	1.200
Rpim	0.102	0.384
Total number of observations	261394	42362
Number of reflections	27532	4387
<I/σ(I)>	8.9	2.6
Completeness [%]	100.0
Redundancy	9.5	9.7
CC(1/2)	0.988	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	2M Ammonium Sulfate