9RC6
Laccase (multicopper oxidase) from Pediococcus pentosaceus 4618 mutant Y471L co-crystallized with Copper Chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 180 |
| Detector technology | PIXEL |
| Collection date | 2024-09-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 123.680, 123.680, 181.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 107.110 - 2.600 |
| R-factor | 0.19128 |
| Rwork | 0.189 |
| R-free | 0.22864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.110 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.116 | 0.987 |
| Rmeas | 0.123 | 1.046 |
| Rpim | 0.039 | 0.344 |
| Total number of observations | 502327 | 41849 |
| Number of reflections | 50107 | 4582 |
| <I/σ(I)> | 13.8 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 10 | 9.1 |
| CC(1/2) | 0.998 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 0.5 M Ammonium Sulfate, 1 M Lithium Sulfate and 100 mM Sodium Citrate pH 5.6 |
