9RC3
Laccase (multicopper oxidase) from Pediococcus pentosaceus 4618 co-crystallized with Silver Nitrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 180 |
| Detector technology | PIXEL |
| Collection date | 2023-11-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.122, 121.122, 174.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 104.890 - 2.700 |
| R-factor | 0.20381 |
| Rwork | 0.202 |
| R-free | 0.24933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.225 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 174.560 | 2.810 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.177 | 1.137 |
| Rmeas | 0.187 | 1.243 |
| Rpim | 0.059 | 0.493 |
| Number of reflections | 41277 | 4547 |
| <I/σ(I)> | 15.9 | 2.4 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 18.5 | 12 |
| CC(1/2) | 0.998 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 25% PEG 4000, 100 mM Sodium Citrate pH 5.6 and 0.2 M Ammonium Sulfate |
