9RC0
DvhD, PAS/HD-GYP domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-06 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 120.931, 120.931, 98.395 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.230 - 3.440 |
| R-factor | 0.317 |
| Rwork | 0.316 |
| R-free | 0.32940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.735 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.230 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.440 |
| Number of reflections | 10703 | 1338 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 21.9 | |
| CC(1/2) | 0.999 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Hepes (pH 7.5), 10% (w/v) polyethylene glycol 6000, and 5% (v/v) MPD |
