9RB4

Ecoli Sarcin Ricin Loop (SLR) including a Kappa-Xanthosine base pair

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-18
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.826561
Spacegroup name	P 43
Unit cell lengths	29.665, 29.665, 76.547
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.660 - 1.000
R-factor	0.1174
Rwork	0.117
R-free	0.13390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.604
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.660	0.974
High resolution limit [Å]	0.938	0.938
Number of reflections	520196	13500
<I/σ(I)>	16.5
Completeness [%]	93.4
Redundancy	12.9
CC(1/2)	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1 volume [3.1M AS, 50mM KMops pH7.0, 10mM MgCl2, 10mM MnCl2] + 2 volumes [RNA 2.5mg/ml, 1mM EDTA pH8.0, 10mM Tris pH 8.0]