Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.550, 80.310, 132.500 |
| Unit cell angles | 91.92, 90.99, 92.41 |
Refinement procedure
| Resolution | 34.890 - 3.000 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.476 |
| Data reduction software | XDS (built 20241002) |
| Data scaling software | XDS (built 20241002) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.890 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.284 | 1.068 |
| Rmeas | 0.307 | 1.156 |
| Rpim | 0.116 | 0.439 |
| Number of reflections | 61337 | 6131 |
| <I/σ(I)> | 6.24 | 1.99 |
| Completeness [%] | 99.8 | 99.82 |
| Redundancy | 6.9 | 6.8 |
| CC(1/2) | 0.990 | 0.512 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12.5% w/v PEG 4.000, 20% w/v 1,2,6-hexanetriol, 100mM BES/TEA pH 7.5, 10mM spermine tetrahydrochloride, 10mM spermidine trihydrochloride, 10mM 1,4-diaminobutane dihydrochloride, 10mM DL-ornithine monohydrochloride, 3% DMSO |
