9R8S
A viral SAVED protein with ring nuclease activity subverts type III CRISPR defence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.614, 110.614, 113.027 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.794 - 1.790 |
| Rwork | 0.165 |
| R-free | 0.20710 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.314 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.930 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.115 | 4.572 |
| Number of reflections | 75593 | 4020 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 38.9 | |
| CC(1/2) | 1.000 | 0.345 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 1.2M sodium phosphate 0.8M potassium phosphate 0.2M lithium phosphate 0.1M CAPS pH 10.5 |
