Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-26 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.678, 71.105, 79.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.650 - 2.040 |
| R-factor | 0.2292 |
| Rwork | 0.227 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.673 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0091) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.680 | 2.160 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmeas | 0.138 | 2.200 |
| Number of reflections | 19561 | 2145 |
| <I/σ(I)> | 12.5 | 1.15 |
| Completeness [%] | 94.4 | |
| Redundancy | 12.9 | |
| CC(1/2) | 0.999 | 0.444 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 0.1M MES pH5.8, 3.5% PEG1000, 25% PEG600, 11% Glycerol |
