9R82
6-Helix Bundle - with a Clasp (6HB-C)-monomer with 2'-Fluoro-modified pyrimidines (FY RNA)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9.33 |
| Software | cryoSPARC,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
