9R73
Pyrin B30.2 domain, FMF mutation delta-Ile692
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 59.540, 59.540, 179.414 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.740 - 1.440 |
| R-factor | 0.17593 |
| Rwork | 0.175 |
| R-free | 0.19711 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.948 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.850 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.130 | 3.125 |
| Rmeas | 0.133 | 3.226 |
| Rpim | 0.028 | 0.772 |
| Total number of observations | 729664 | 26458 |
| Number of reflections | 35210 | 1690 |
| <I/σ(I)> | 15 | 1 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.7 | 15.7 |
| CC(1/2) | 0.999 | 0.407 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-AcOH, pH 7.6; 0.2 M KSCN; 30 % (w/v) PEG 2k MME |
