9R70
Pyrin B30.2 domain, FMF mutation M680L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.364, 60.231, 84.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.960 - 1.500 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.21205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.877 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.960 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.101 | 1.439 |
| Rmeas | 0.106 | 1.666 |
| Rpim | 0.032 | 0.818 |
| Total number of observations | 335757 | 3835 |
| Number of reflections | 32908 | 937 |
| <I/σ(I)> | 14.2 | 0.9 |
| Completeness [%] | 93.6 | |
| Redundancy | 10.2 | 4.1 |
| CC(1/2) | 0.999 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-AcOH, pH 8.0; 0.2 M KSCN; 23.3 % (w/v) PEG 2k MME |
