9R4G
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: T4 Lysozyme, mutant L99A bound with indole (1 s soaking)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.173, 60.173, 97.217 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.170 - 1.900 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.24867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.785 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.170 | 2.008 |
| High resolution limit [Å] | 1.896 | 1.896 |
| Number of reflections | 11696 | 586 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 87.4 | |
| Redundancy | 18.3 | |
| CC(1/2) | 0.992 | 0.517 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 293 | 4 M sodium/potassium phosphate pH 7.0, 0.1 M 1,6-hexanediol, 0.15 M NaCl |
