9R4F

Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: T4 Lysozyme, mutant L99A (apo state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.976
Spacegroup name	P 32 2 1
Unit cell lengths	60.097, 60.097, 96.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.100 - 1.500
R-factor	0.17771
Rwork	0.177
R-free	0.19356
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.823
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.046	1.567
High resolution limit [Å]	1.497	1.497
Number of reflections	29721	1383
<I/σ(I)>	12.4
Completeness [%]	94.5
Redundancy	18.52
CC(1/2)	0.998	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293	4 M sodium/potassium phosphate pH 7.0, 0.1 M 1,6-hexanediol, 0.15 M NaCl