9R48

Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Human insulin, pH 9.0 (apo state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-08
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	I 21 3
Unit cell lengths	78.802, 78.802, 78.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.430 - 1.650
R-factor	0.18653
Rwork	0.186
R-free	0.19625
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.999
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.401	1.736
High resolution limit [Å]	1.650	1.650
Number of reflections	8996	450
<I/σ(I)>	9.9
Completeness [%]	94.1
Redundancy	7.92
CC(1/2)	0.996	0.541

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	9	293	1.0 M sodium/potassium phosphate, pH 9.0