9R35

Crystal structure of the Pseudomonas putida Xre-RES toxin-antitoxin complex bound to promoter DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	81.230, 185.110, 170.790
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	26.030 - 2.700
Rwork	0.228
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (2.8.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.030	26.030	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.091	0.040	1.393
Rmeas	0.108	0.047	1.687
Number of reflections	139781	1674	6309
<I/σ(I)>	8.13
Completeness [%]	90.3
Redundancy	3.4
CC(1/2)	0.997	0.996	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292.15	0.1 M Na-HEPES, pH 7.5, 10% (w/v) PEG 8000, 8% (v/v) ethylenglycol, and 0.2 M NaCl