9R0Z

De novo designed homodimeric NTF2-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97926
Spacegroup name	P 2 21 21
Unit cell lengths	37.321, 37.883, 68.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.320 - 2.000
R-factor	0.2162
Rwork	0.212
R-free	0.25736
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.880	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	18616	504
<I/σ(I)>	31.7
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	1.000	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	23 % peg 3350 , Zinc acetate 0.05M