9R0T

De novo designed homodimeric NTF2-like protein with a hydrophobic pocket

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	149.680, 39.262, 95.261
Unit cell angles	90.00, 120.61, 90.00

Refinement procedure

Resolution	71.360 - 1.900
R-factor	0.2033
Rwork	0.202
R-free	0.23876
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.980	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	38057	2444
<I/σ(I)>	14.1
Completeness [%]	99.9
Redundancy	6.1
CC(1/2)	0.990	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	23 % peg 3350 0.05M Zinc Acetate