9R08
Structure of the human heterotetrameric cis-prenyltransferase complex harboring NgBR-S249A in complex with magnesium, FsPP and IPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-02 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 184.399, 184.399, 112.575 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.100 - 2.400 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.603 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.181 | 2.632 |
| Number of reflections | 55744 | 5521 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 292 | 0.1 M Tris pH 9, 1 M Glycine, 0.1M KCl, 41% (v/v) PEG 400 |
