9R00
De novo NTF2-like protein with a 2-helix C-terminal extension.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.397, 61.043, 96.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.100 - 2.000 |
| R-factor | 0.22026 |
| Rwork | 0.216 |
| R-free | 0.26391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 8367 | 599 |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 27% peg 400, cadmiun chloride 0.1M Sodium acetate 100 mM pH 4.6 |
