9QZD

de novo NTF2-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	36.829, 62.021, 80.443
Unit cell angles	90.00, 102.50, 90.00

Refinement procedure

Resolution	78.540 - 2.400
R-factor	0.2245
Rwork	0.223
R-free	0.25607
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.540	2.462
High resolution limit [Å]	2.400	2.400
Rmerge		1.524
Number of reflections	12468	1023
<I/σ(I)>	6.8
Completeness [%]	93.6
Redundancy	1.6
CC(1/2)	0.999	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	27% PEG 3350, 0.1M Bis-Tris pH 5.4