9QYO

Structural characterization of a mannose-binding site in human RNase2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	41.806, 52.576, 56.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.680 - 1.020
R-factor	0.1335
Rwork	0.133
R-free	0.14870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.791
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.680	1.050
High resolution limit [Å]	1.020	1.020
Rmerge	0.063	0.094
Number of reflections	49099	345
<I/σ(I)>	6.2	1.02
Completeness [%]	76.4
Redundancy	4.1
CC(1/2)	0.992	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.18M Sodium Formate, 0.1M Bis-Tris propane, 15% PEG 3350, PH=8.2