9QWO
Vinculin tail bound to paxillin LD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979264 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.382, 70.562, 117.372 |
| Unit cell angles | 90.00, 131.25, 90.00 |
Refinement procedure
| Resolution | 44.870 - 2.540 |
| R-factor | 0.22675 |
| Rwork | 0.225 |
| R-free | 0.25909 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.870 | 2.690 |
| High resolution limit [Å] | 2.540 | 2.550 |
| Rmerge | 0.102 | 0.856 |
| Rmeas | 0.124 | 1.035 |
| Rpim | 0.069 | 0.575 |
| Total number of observations | 16149 | |
| Number of reflections | 35621 | 5168 |
| <I/σ(I)> | 7.3 | 1.4 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.995 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 1.26 M (NH4)2SO4, 0.2 M NaCl, 0.1 M Na Acetate pH4.5 |
