9QUF
Structure of a fungal Ube2O
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 61 |
| Unit cell lengths | 154.010, 154.010, 90.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.460 - 3.000 |
| R-factor | 0.2315 |
| Rwork | 0.229 |
| R-free | 0.28740 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data scaling software | XSCALE |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 48136 | 3369 |
| <I/σ(I)> | 29.4 | 4.91 |
| Completeness [%] | 99.5 | |
| Redundancy | 10.3 | |
| CC(1/2) | 1.000 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 5% (w/v) PEG8000, 1M Li2SO4 |
