9QQN

Junin virus GP1-GP2 heterodimer in complex with Fab of JUN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	C 1 2 1
Unit cell lengths	226.468, 73.030, 80.734
Unit cell angles	90.00, 95.78, 90.00

Refinement procedure

Resolution	80.320 - 2.550
R-factor	0.2029
Rwork	0.201
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.584
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.660	2.590
High resolution limit [Å]	2.550	2.550
Number of reflections	43012	2206
<I/σ(I)>	7.2
Completeness [%]	99.9
Redundancy	4
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.5 mM Yttrium(III) chloride hexahydrate, 0.5 mM Erbium(III) chloride hexahydrate, 0.5 mM Terbium(III) chloride hexahydrate, 0.5 mM Ytterbium(III) chloride hexahydrate 0.1 M MOPSO/Bis-Tris, pH 6.5 10% w/v PEG 8000, 20% v/v 1,5-Pentanediol